Nitrile reductase (NRED)
Description:
De Nitrile reductase
Genus NRED enzyme productum est (Number sicut ES-NRED101~ES-NRED103) per SyncoZymes evolvit.NRED catalyse reductionem globi nitrilis ad amina.In processu catalysis NADPH opus est ut transportator hydrogenii.
Genus reactionis nitrile reductasecatalytic
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| Enzymes | Product Code | Specification |
| Specification | ES- NRED -101~ ES- NRED -103 | statuto nitrili reductase 3 , 50 mg quaelibet 3 items * 50 mg / item , vel alia quantitas |
Hoc reactionem ratio potest adhiberi ut referat:
1-10 mg/ml Substratum
10 mg/ml Enzyme
10% (v/v) Organicum cosolvendum (1)
100 mM quiddam Phosphate (pH7.0)
0.2 mg/ml NADP+
10 mg/ml Glucosum
2 mg/ml Glucosum dehydrogenas (GDH)
Incubant motus constanti temperie concussoris (exempli gratia 30oC, 150rpm) in 24~48 horis.Centrifugium quodlibet mixtum ad interdum praecipitandum, illa supernatans adhiberi potest pro analysis TLC、HPLC vel GC.
Nota (1): solvens organica (qualia DMSO, methanolum, acetonitrilum, IPA, etc.) est libitum, adhibitum pro substratis insolubilibus.
