Leucine Dehydrogenase (LeuDH)
Description:
About Leucine dehydrogenase
Genus LeuDH enzyme productum est (Number sicut ES-LeuDH-101) evolvit SyncoZymes.LeuDH catalysem ammoniationem 4-methylbutanoici acidi (salis) ad L-leucinam vel oxidationem leucinium ad 4-methylbutanoicum acidum cataly.ES-LeuDH potest etiam aminationem reductivam acida α-keto cum simili structura adaequare derivationibus amino acidis correspondentibus praeparare.In processu catalysis NADH necessaria est ut transportator hydrogenii.
Leucine dehydrogenasecatalytic reaction type
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| Enzymes | Product Code | Specification |
| Enzyme Pulvis | ES-LeuDH-101 | a paro of 1 Monoamine Oxidase, 50 mg singulorum 1 item * 50 mg / item, vel alia quantitas |
Hoc reactionem ratio potest adhiberi ut referat:
1-10 mg/ml: Substratum
10 mg/ml: Enzyme
10% (v/v): Organicum cosolvendum (1)
100 mM: Phosphate quiddam (pH7.0)
0.2 mg/ml: NAD+
100 mM: Aconitum formate
10 mg/ml: dehydrogenas formate (FDH)
Incubant motus constanti temperie concussoris (exempli gratia 30oC, 150rpm) in 24~48 horis.Centrifugium quodlibet mixtum ad interdum praecipitandum, illis supernatans adhiberi potest pro analysis TLC, HPC vel GC.
Nota (1): solvens organica (qualia DMSO, methanolum, acetonitrilum, IPA, etc.) est libitum, adhibitum pro substratis insolubilibus.
