Hydroaminase (HAM)
Description:
De Hydroaminase
Enzyme productum genera II sunt (Number sicut ES-HAM-101~ ES-HAM-102) a SyncoZymes evolvit.HAM ammoniationem acidi enoici vel eius derivationes ad acida chiralorum amino catalystare potest.HAM adhiberi potest ad acida chiralalia amino (vel aminos chirales) componi ab acida enoica (vel alkenes).Ammonia donator, ut aqua ammonia, vel salis ammonium, necessarium est.
Hydroaminase catalytic genus reactionem
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| Enzymes | Product Code | Specification |
| Enzyme Pulvis | ES-HAM-101~ES-HAM-102 | a paro of 2 Nuclosidephosphoryalse, 50 mg singulorum 2 items * 50mg / item, vel alia quantitas. |
Hoc reactionem ratio potest adhiberi ut referat:
1-10 mg/ml: Substratum
10 mg/ml: Enzyme
10% (v/v): Organicum cosolvendum (1)
10% (v/v): Ammonia
pH ad 9.0 compone antequam enzyme addatur, et enzyme tandem adiciatur.Incubant motus constanti temperie concussoris (exempli gratia 30oC, 150rpm) in 24~48 horis.Centrifugium quodlibet mixtum ad interdum praecipitandum, illa supernatans adhiberi potest pro analysis TLC、HPLC vel GC.
Nota (1): solvens organica (qualia DMSO, methanolum, acetonitrilum, IPA, etc.) est libitum, adhibitum pro substratis insolubilibus.
